القائمة
Order DNA synthesis from Twist Bioscience. Use when the user asks to order clonal genes, gene blocks, or gene fragments from Twist, or to submit codon-optimized sequences for synthesis.
التثبيت باستخدام Codex أو Claude انسخ هذا Prompt والصقه في Codex أو Claude أو مساعد آخر ليراجع صفحة Skill ويثبّتها لك.
استنادا إلى تصنيف SOC المهني
Predict optimal construct boundaries for protein expression. Use when the user asks about domain boundaries, construct design, truncations, which region of a protein to express, or identifying disordered regions.
Manage lab notebook entries, registry items (plasmids, proteins, etc.), templates, widgets, and audit logs via the Labbook service. Use when creating or viewing lab notebook entries, managing the sample registry, or documenting experiments.
Verify open reading frames (ORFs) in plasmid sequences. Use when the user asks about verifying protein coding sequences, checking if an ORF is present in a plasmid, finding where a protein is encoded, or doing six-frame translation analysis.
Fetch sequencing data from Plasmidsaurus. Use when the user asks about listing sequencing orders, downloading sequencing results, checking order status, or retrieving plasmid sequences from Plasmidsaurus.
Design PCR primers for molecular cloning. Use when the user asks about designing primers, LIC cloning primers, sequencing primers, Gibson assembly primers, or working with NCBI accession codes for cloning.
Calculate protein parameters (MW, pI, extinction coefficient) and generate purification recommendations. Use when the user asks about protein properties, molecular weight, isoelectric point, extinction coefficients, or purification strategies.
| name | twist-order |
| description | Order DNA synthesis from Twist Bioscience. Use when the user asks to order clonal genes, gene blocks, or gene fragments from Twist, or to submit codon-optimized sequences for synthesis. |
Order clonal genes, gene blocks, and gene fragments via Twist Bioscience API.
twist_order
Set in .env:
TWIST_API_TOKEN - Twist API tokenTWIST_USER_EMAIL - Twist account emailtwist_order gene \
--sequence <DNA> \
--name <NAME> \
--vector-id <VECTOR_MES_UID> \
--insertion-point-id <INSERTION_POINT_MES_UID> \
--recipient-address-id <ADDRESS_ID> \
--first-name <FIRST> \
--last-name <LAST> \
--phone <PHONE> \
--payment-method-id <PAYMENT_ID>
twist_order gene-block \
--sequence <DNA> \
--name <NAME> \
--recipient-address-id <ADDRESS_ID> \
--first-name <FIRST> \
--last-name <LAST> \
--phone <PHONE> \
--payment-method-id <PAYMENT_ID>
twist_order vectors list
| Option | Description |
|---|---|
--sequence | DNA sequence (A/C/T/G/N only) |
--sequence-file | Path to file with DNA sequence (FASTA OK) |
--name | Construct name |
--vector-id | Vector MES UID (clonal genes only) |
--insertion-point-id | Insertion point MES UID (clonal genes only) |
--adapters-on | Enable adapters for gene fragments |
--glycerol-stock | Add glycerol stock (clonal genes) |
--quote-only | Create quote only, don't place order |
--no-po | Order without PO (if allowed) |
| Option | Default | Description |
|---|---|---|
--dna-scale | GENE_PREP_MICRO | DNA scale product code |
--delivery-format | SER_PKG_TUBE | Tube or plate format |
--buffer-code | (none) | Optional buffer product code |
--normalization | 0 | Normalization value (0.5-2.0) |
Codon optimize (IDT):
python3 scripts/codon_optimize_cli.py --accession NP_003161 --organism insect --fasta > spt6_opt.fa
Get quote (Twist):
twist_order gene \
--sequence-file spt6_opt.fa \
--name SUPT6H_insect \
--vector-id <from vectors list> \
--insertion-point-id <from vectors list> \
--recipient-address-id <your address> \
--first-name <FIRST> --last-name <LAST> \
--phone <phone> \
--quote-only
Place order (remove --quote-only, add --payment-method-id)
vectors list)--quote-only to review pricing before ordering