| name | benchos-complex-formation |
| description | Assemble and purify macromolecular complexes for structural biology. Use when the user asks about SEC complex assembly, stoichiometry, expected elution positions, A260/A280 interpretation, or fraction-selection strategy. |
| license | Proprietary |
| compatibility | Requires BenchOS agent runtime with `labbook_cli`; use BenchOS compute skills separately when calculations are needed. |
| metadata | {"author":"benchos","version":"1"} |
| allowed-tools | labbook_cli |
BenchOS Complex Formation
Use this skill for assembly and SEC-purification guidance for macromolecular complexes.
Core Rules
- Always compare SEC elution position against empirical expectations for the target complex size.
- Always run SDS-PAGE on candidate fractions before committing downstream.
- Prefer single, well-behaved fractions over pooled material for grid preparation.
- Treat unexpectedly high A260 or shifted A260/A280 as a complex-composition warning, not noise.
BenchOS Workflow
- Query BenchOS stocks or registry items for current component concentrations.
- Calculate stoichiometry and setup volumes with
benchos-reactor if the user needs a recipe.
- Evaluate SEC trace shape, elution position, and A260/A280.
- Record the chosen fraction and rationale in the relevant BenchOS entry.
