| name | benchos-insect-cell |
| description | Baculovirus expression system guidance for recombinant protein production. Use when the user asks about Sf9, Sf21, Hi5, bacmid generation, V0/V1 production, infection strategy, or insect-cell expression troubleshooting. |
| license | Proprietary |
| compatibility | Requires BenchOS agent runtime with `labbook_cli`; this is a guidance skill. |
| metadata | {"author":"benchos","version":"1"} |
| allowed-tools | labbook_cli |
BenchOS Insect Cell
Use this skill for baculovirus-based protein-expression guidance.
Core Rules
- Keep bacmid DNA handling gentle; do not use workflows that shear large bacmids.
- Use white-colony selection and YFP tracking as core checkpoints in the workflow.
- Choose Hi5 for yield when appropriate, but switch cell line based on empirical target behavior.
- Treat V0, V1, and large-scale expression as separate optimization stages.
BenchOS Workflow
- Confirm the construct and vector strategy.
- Plan bacmid generation, transfection, and V0/V1 amplification.
- Choose the large-scale expression cell line and infection plan.
- Record virus batch, infection timing, and harvest criteria in BenchOS entries.
