القائمة
Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.
التثبيت باستخدام Codex أو Claude انسخ هذا Prompt والصقه في Codex أو Claude أو مساعد آخر ليراجع صفحة Skill ويثبّتها لك.
استنادا إلى تصنيف SOC المهني
Use when an agent needs to call the VirusPrimerPro Agent API instead of the GUI, or prepare viral genome inputs for it: request/use API keys, download viral genomes from NCBI, install/run MAFFT, check and repair abnormal sequences, prepare phylogenetic trees/annotations, upload aligned FASTA/tree/GenBank files, poll runs, and fetch artifacts.
Use when an agent needs a stricter or publication-grade phylogeny workflow for PhyloGuide, including tree/MSA validation, FastTree/IQ-TREE planning, and external-tree checks.
Use when an agent must define viral genotype, subtype, clade, lineage, target/background groups, or PhyloGuide groups from cited literature before primer or tNGS panel design.
Use when an agent must split tNGS or multiplex primer pairs into pools while minimizing primer-primer interaction risk and reporting pool overflows.
Use when an agent must select, filter, deduplicate, and document NCBI/GenBank/reference viral genomes before alignment, primer design, PhyloGuide, or tNGS panel design.
Use when an agent must batch-submit, poll, summarize, retry, or compare long-running VirusPrimerPro Agent API jobs without holding a long HTTP connection open.
| name | primer-wetlab-qc |
| description | Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size. |
Use this skill after primer_site artifacts or external candidate generation. The bundled helper is dependency-free and conservative; final release should also run Primer3/ntthal or an equivalent thermodynamic tool when installed.
python agentskill/scripts/primer_panel_qc.py qc-primers \
--primers primer_pairs.csv \
--output primer_qc.json \
--csv-output primer_qc.csv \
--min-primer-len 18 \
--max-primer-len 30 \
--min-tm 50 \
--max-tm 68 \
--max-degeneracy 16
Review all pairs with:
degeneracy_too_high;three_prime_*_complement_high;pair_complement_run_high;amplicon_length_out_of_range;Treat primer_qc.json as a design gate before specificity screening and panel export.