fluorescence-spectroscopy-analyzer

name	fluorescence-spectroscopy-analyzer
description	Fluorescence spectroscopy skill for quantum dot characterization, FRET measurements, and photoluminescence analysis
allowed-tools	["Read","Write","Glob","Grep","Bash"]
metadata	{"specialization":"nanotechnology","domain":"science","category":"spectroscopy","priority":"high","phase":6,"tools-libraries":["FluorEssence","Edinburgh Instruments F980","Custom analysis tools"]}
graph	{"domains":["domain:nanotechnology"],"skillAreas":["skill-area:mathematical-reasoning","skill-area:physics-simulation","skill-area:data-analysis"],"workflows":["workflow:experiment-design"],"roles":["role:research-engineer"]}

Fluorescence Spectroscopy Analyzer

Purpose

The Fluorescence Spectroscopy Analyzer skill provides comprehensive photoluminescence characterization of nanomaterials, enabling analysis of emission properties, quantum yields, and energy transfer processes.

Capabilities

• Excitation/emission spectra
• Quantum yield calculation
• Fluorescence lifetime measurements
• FRET efficiency calculation
• Photostability assessment
• Single-particle fluorescence

Usage Guidelines

Fluorescence Analysis

1. Spectral Measurements

   • Acquire excitation and emission spectra
   • Correct for instrument response
   • Identify emission maxima

2. Quantum Yield

   • Use integrating sphere or reference
   • Account for reabsorption
   • Report with standard

3. Lifetime Measurements

   • Use TCSPC for time-resolved
   • Fit decay curves
   • Identify multiple components

Process Integration

• Multi-Modal Nanomaterial Characterization Pipeline
• Nanosensor Development and Validation Pipeline
• Nanoparticle Drug Delivery System Development

Input Schema

{
  "sample_id": "string",
  "excitation_wavelength": "number (nm)",
  "measurement_type": "steady_state|time_resolved|quantum_yield",
  "reference_standard": "string (optional)"
}

Output Schema

{
  "emission": {
    "peak_wavelength": "number (nm)",
    "fwhm": "number (nm)",
    "stokes_shift": "number (nm)"
  },
  "quantum_yield": {
    "value": "number",
    "method": "string",
    "reference": "string"
  },
  "lifetime": {
    "tau1": "number (ns)",
    "amplitude1": "number",
    "tau2": "number (ns, optional)",
    "chi_squared": "number"
  }
}