| id | acquire_4d_stem |
| name | Acquire 4D-STEM on SEM |
| description | Workflow for starting BF preview, waiting for preview stabilization, synchronizing pixel size, confirming ROI, stopping preview, waiting, and acquiring 4D-STEM data. |
| version | 1.3.0 |
Agents Involved
Required Tools
agent_sem.play_bf with arguments {} to start BF preview.agent_sem.sync_pixel_size with arguments {} to synchronize pixel size from BF preview and recalculate ROI position/size fields.agent_sem.wait with arguments {"seconds": <integer>} for user confirmation gates or required delays.agent_sem.stop_playing_detector with arguments {} to stop BF preview before acquisition.agent_sem.acquire_4d_stem with arguments {"defocus_A": <number|null>, "dwell_time": <number|null>, "scan_points_x": <integer|null>, "scan_points_y": <integer|null>, "scan_step_x_A": <number|null>, "scan_step_y_A": <number|null>}.
Steps
- Start BF preview: Call
agent_sem.play_bf.
- Wait for BF preview stabilization: Immediately after
agent_sem.play_bf succeeds, call agent_sem.wait with arguments {"seconds": 10}. This 10 second wait is mandatory before pixel-size synchronization.
- Synchronize pixel size: Call
agent_sem.sync_pixel_size. This must target the BF preview data item, update BF preview calibration, and recalculate ROI position/size fields in the UI.
- Ask user to confirm ROI selection: After
agent_sem.sync_pixel_size succeeds, pause and ask the user to confirm that the ROI shown on BF preview is the intended scan region. Represent this as a PlanStep using agent_sem.wait with arguments {"seconds": 1} and put confirm_roi_selection_after_pixel_sync in required_inputs. Do not proceed until the user confirms.
- Stop BF preview: Call
agent_sem.stop_playing_detector.
- Wait after stopping preview: Immediately after
agent_sem.stop_playing_detector succeeds, call agent_sem.wait with arguments {"seconds": 10}. This 10 second wait is mandatory.
- Acquire Data: Call
agent_sem.acquire_4d_stem. For scan grid, one X-direction value (scan_points_x or scan_step_x_A) and one Y-direction value (scan_points_y or scan_step_y_A) are sufficient. If user did not provide scan grid values, call with JSON null or omit those keys so the tool checks existing UI fields. If neither chat input nor UI contains one value per direction, the tool will ask for the missing X/Y scan grid input. The UI auto-calculates the paired value from ROI size when one value per direction is updated.
Planning Contract
- The planner must not add steps outside the seven steps above.
- For event-triggered continuation, if UI action context shows that one or more earlier steps already completed successfully, omit those completed steps and plan only the remaining suffix of the seven-step workflow. Keep the original logical step order, but the PlanReview may start at the next incomplete step.
- The planner must use the exact tool names above. Do not use old names such as
sem_agent, turn_on_inlens_detector, confirm_roi_selection, apply_roi_parameters, calculate_scan_parameters, or acquire_4d_stem_data.
- Do not call
agent_sem.get_sem_state in this workflow unless the user explicitly asks to inspect SEM state as a separate request.
- Do not call
agent_sem.calculate_scan_parameters in this workflow. The UI already performs scan-grid auto-calculation when scan points or scan step fields are updated.
- The only ROI confirmation is the chat/user-confirmation gate immediately after
agent_sem.sync_pixel_size; do not use or invent a ROI confirmation tool.
- The wait after starting BF preview must be exactly
agent_sem.wait with arguments {"seconds": 10} before agent_sem.sync_pixel_size.
- The wait after stopping BF preview must be exactly
agent_sem.wait with arguments {"seconds": 10}.
- Optional numeric arguments must be JSON numbers when known and JSON
null or omitted when unknown. Do not use the string "None".
- Do not add skip-acquisition, existing-data checks, SEM state checks, final validation, reconstruction, or parameter recommendation steps unless the user explicitly selects another skill that contains those steps.
list_agents_capabilities may be used only to verify that the required tools exist; it must not be used to add extra tools to this workflow.
Completed Action Mapping
Use these UI/action events as evidence of completed workflow steps:
agent_sem.trigger_llm play_bf, tool_name=play_bf, or "BF Preview playback started" means step 1 is complete; the next planned step should be step 2 (agent_sem.wait {"seconds": 10}).agent_sem.trigger_llm sync_pixel_size, tool_name=sync_pixel_size, or "Pixel size sync" means step 3 is complete; the next planned step should be step 4, asking the user to confirm ROI selection.agent_sem.trigger_llm stop_bf, tool_name=stop_bf, tool_name=stop_playing_detector, or "BF Preview playback stopped" means step 5 is complete; the next planned step should be step 6 (agent_sem.wait {"seconds": 10}).agent_sem.trigger_llm acquire_4d_stem with an acquisition-started or acquisition-finished message means step 7 has started or completed; do not plan a duplicate acquisition unless the user explicitly asks for another run.
Success Criteria
- BF preview starts.
- 10 seconds are waited after starting BF preview and before pixel-size synchronization.
- Pixel size is synchronized from BF preview and ROI position/size fields are recalculated.
- User confirms the BF preview ROI after pixel-size synchronization.
- BF preview is stopped.
- 10 seconds are waited after stopping BF preview.
- 4D-STEM acquisition returns a saved data path or success message.
Failure Policy
- If pixel size synchronization, ROI confirmation, defocus, dwell, or scan grid inputs are missing, put them in the relevant step's
required_inputs list and use on_failure: ask_user; do not block the whole plan with PlanReview missing_inputs.
- If any microscope tool fails, stop the workflow unless the user provides recovery instructions.
