benchos-insect-cell

name	benchos-insect-cell
description	Baculovirus expression system guidance for recombinant protein production. Use when the user asks about Sf9, Sf21, Hi5, bacmid generation, V0/V1 production, infection strategy, or insect-cell expression troubleshooting.
license	Proprietary
compatibility	Requires BenchOS agent runtime with `labbook_cli`; this is a guidance skill.
metadata	{"author":"benchos","version":"1"}
allowed-tools	labbook_cli

BenchOS Insect Cell

Use this skill for baculovirus-based protein-expression guidance.

Core Rules

• Keep bacmid DNA handling gentle; do not use workflows that shear large bacmids.
• Use white-colony selection and YFP tracking as core checkpoints in the workflow.
• Choose Hi5 for yield when appropriate, but switch cell line based on empirical target behavior.
• Treat V0, V1, and large-scale expression as separate optimization stages.

BenchOS Workflow

1. Confirm the construct and vector strategy.
2. Plan bacmid generation, transfection, and V0/V1 amplification.
3. Choose the large-scale expression cell line and infection plan.
4. Record virus batch, infection timing, and harvest criteria in BenchOS entries.