protocol-writing

name	protocol-writing
description	null

Protocol Writing — Reproducible Lab Protocols & SOPs

Overview

Write clear, reproducible experimental protocols and Standard Operating Procedures (SOPs) for any scientific discipline.

Structure

1. Title and Version — Protocol name, version number, date, author
2. Purpose — What this protocol achieves and when to use it
3. Safety — Hazards, PPE requirements, waste disposal
4. Materials — Exact reagents (catalog numbers, lot numbers), equipment, consumables
5. Preparation — Buffer recipes, stock solutions, equipment setup
6. Procedure — Numbered steps with exact quantities, temperatures, times, speeds
7. Quality Control — Expected results, acceptance criteria, troubleshooting
8. Data Recording — What to record, where, in what format
9. References — Published methods this protocol is based on

Key Principles

• Exact quantities: "Add 2.5 mL" not "add some"
• Catalog numbers: Every reagent identified by manufacturer and catalog number
• Time precision: "Incubate for 30 min at 37°C" not "incubate for a while"
• Critical steps: Mark steps where deviation causes failure
• Troubleshooting table: Common problems and solutions
• Version control: Track changes between protocol versions