菜单
End-to-end pooled and single-cell CRISPR screen pipeline: library validation, guide counting, six-stage QC, copy-number/batch correction, design-matched hit calling, and tier-based consensus.
用 Codex 或 Claude 帮你安装 复制这段 Prompt，粘贴到 Codex、Claude 或其他助手里，让它检查 Skill 页面并帮你完成安装。
基于 SOC 职业分类
Load BioMaster as one integrated skill-driven bioinformatics assistant.
BioMaster conversational bioinformatics assistant; starts workflows only after execution intent or empty activation.
Automated and marker-guided single-cell cell type annotation using CellTypist, marker review, reference transfer, and confidence-aware label curation.
Ligand-receptor communication inference in single-cell or spatial data with sender-receiver summaries and cautious interpretation.
Workflow for paired or integrated single-cell RNA and ATAC analysis with multimodal latent spaces and regulatory interpretation.
Workflow for spatial transcriptomics preprocessing, domain detection, deconvolution, neighborhood analysis, and publication-ready spatial maps.
| name | bioskills-crispr-screen-pipeline |
| description | End-to-end pooled and single-cell CRISPR screen pipeline: library validation, guide counting, six-stage QC, copy-number/batch correction, design-matched hit calling, and tier-based consensus. |
| domain | single-cell |
| primary_tool | MAGeCK |
| methods | ["MAGeCK","BAGEL2","drugZ","JACKS","Chronos","CRISPRcleanR","MAGeCKFlute","pertpy"] |
| macro_tags | ["crispr-screen","pooled-screen","hit-calling","quality-control","consensus","workflow"] |
| micro_tags | ["mageck","bagel2","drugz","jacks","chronos","crisprcleanr","mageckflute","gini","pr-auc","copy-number","batch-correction","volcano"] |
| source | bioskills-crispr-screen-pipeline |
| status | active |
| enabled | true |
