菜单
Use when an agent must turn primer pair candidates into a tiled or multiplex tNGS amplicon panel with target coverage, overlap, selected pairs, and uncovered gap reporting.
用 Codex 或 Claude 帮你安装 复制这段 Prompt，粘贴到 Codex、Claude 或其他助手里，让它检查 Skill 页面并帮你完成安装。
基于 SOC 职业分类
| name | tngs-panel-design |
| description | Use when an agent must turn primer pair candidates into a tiled or multiplex tNGS amplicon panel with target coverage, overlap, selected pairs, and uncovered gap reporting. |
Use this skill when the deliverable is a panel rather than one diagnostic amplicon. Inputs should already be curated, aligned, and primer-QC checked.
Candidate CSV/JSON must include pair_id, forward_sequence, reverse_sequence, amplicon_start, and amplicon_end.
python agentskill/scripts/primer_panel_qc.py design-panel \
--candidates candidate_pairs.csv \
--genome-length 30000 \
--required-overlap 20 \
--min-coverage 0.95 \
--output panel_plan.json \
--csv-output selected_panel.csv
coverage_fraction and every gap in panel_plan.json.Use when an agent needs to call the VirusPrimerPro Agent API instead of the GUI, or prepare viral genome inputs for it: request/use API keys, download viral genomes from NCBI, install/run MAFFT, check and repair abnormal sequences, prepare phylogenetic trees/annotations, upload aligned FASTA/tree/GenBank files, poll runs, and fetch artifacts.
Use when an agent needs a stricter or publication-grade phylogeny workflow for PhyloGuide, including tree/MSA validation, FastTree/IQ-TREE planning, and external-tree checks.
Use when an agent must define viral genotype, subtype, clade, lineage, target/background groups, or PhyloGuide groups from cited literature before primer or tNGS panel design.
Use when an agent must split tNGS or multiplex primer pairs into pools while minimizing primer-primer interaction risk and reporting pool overflows.
Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.
Use when an agent must select, filter, deduplicate, and document NCBI/GenBank/reference viral genomes before alignment, primer design, PhyloGuide, or tNGS panel design.