القائمة
Use when an agent must screen primer candidates or tNGS panel primers for host, near-neighbor, background, or co-infection off-target binding before reporting or ordering primers.
التثبيت باستخدام Codex أو Claude انسخ هذا Prompt والصقه في Codex أو Claude أو مساعد آخر ليراجع صفحة Skill ويثبّتها لك.
استنادا إلى تصنيف SOC المهني
Use when an agent needs to call the VirusPrimerPro Agent API instead of the GUI, or prepare viral genome inputs for it: request/use API keys, download viral genomes from NCBI, install/run MAFFT, check and repair abnormal sequences, prepare phylogenetic trees/annotations, upload aligned FASTA/tree/GenBank files, poll runs, and fetch artifacts.
Use when an agent needs a stricter or publication-grade phylogeny workflow for PhyloGuide, including tree/MSA validation, FastTree/IQ-TREE planning, and external-tree checks.
Use when an agent must define viral genotype, subtype, clade, lineage, target/background groups, or PhyloGuide groups from cited literature before primer or tNGS panel design.
Use when an agent must split tNGS or multiplex primer pairs into pools while minimizing primer-primer interaction risk and reporting pool overflows.
Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.
Use when an agent must select, filter, deduplicate, and document NCBI/GenBank/reference viral genomes before alignment, primer design, PhyloGuide, or tNGS panel design.
| name | specificity-screening |
| description | Use when an agent must screen primer candidates or tNGS panel primers for host, near-neighbor, background, or co-infection off-target binding before reporting or ordering primers. |
Use this skill after candidate primer generation and before wet-lab export. Production specificity should use BLAST/Bowtie2 databases when available; the bundled helper provides a deterministic local FASTA screen for smoke tests and small curated backgrounds.
python agentskill/scripts/specificity_screen.py kmer-screen \
--primers primer_pairs.csv \
--background-fasta background.fasta \
--max-mismatches 1 \
--seed-length 10 \
--output specificity_report.json
Use --fail-on-hit when any background hit should block release.
Do not call a primer production-ready if only target coverage has been checked.