flow-cytometry-gating-strategist

name	flow-cytometry-gating-strategist
description	Recommend optimal flow cytometry gating strategies for specific cell types and fluorophores
version	1.0.0
category	Bioinfo
tags	[]
author	AIPOCH
license	MIT
status	Draft
risk_level	High
skill_type	Hybrid (Tool/Script + Network/API)
owner	AIPOCH
reviewer
last_updated	2026-02-06

Skill: Flow Cytometry Gating Strategist

Recommend optimal flow cytometry gating strategies for given cell types and fluorophores.

Basic Information

• ID: 103
• Name: Flow Cytometry Gating Strategist
• Purpose: Flow cytometry data analysis and gating strategy recommendations

Usage

Command Line

# Recommended format: comma-separated cell types and fluorophores
python scripts/main.py "CD4+ T cells,CD8+ T cells" "FITC,PE,APC"

# Or specify parameters separately
python scripts/main.py --cell-types "CD4+ T cells,CD8+ T cells" --fluorophores "FITC,PE,APC"

# Support more options
python scripts/main.py \
  --cell-types "B cells" \
  --fluorophores "FITC,PE,PerCP-Cy5.5,APC" \
  --instrument "BD FACSCanto II" \
  --purpose "cell sorting"

Parameters

Parameter	Type	Default	Required	Description
--cell-types	string	-	Yes	Comma-separated list of cell types (e.g., "CD4+ T cells,CD8+ T cells")
--fluorophores	string	-	Yes	Comma-separated list of fluorophores (e.g., "FITC,PE,APC")
--instrument	string	-	No	Flow cytometer model (e.g., "BD FACSCanto II")
--purpose	string	analysis	No	Purpose (analysis, cell sorting, screening)
--output, -o	string	stdout	No	Output file path for JSON results

Output Format

{
  "recommended_strategy"