biotechnology

## What I do - Develop biological processes and products using living organisms - Apply genetic engineering and molecular biology techniques - Design bioprocesses for industrial production - Create recombinant proteins and therapeutics - Develop diagnostic tests and biosensors - Engineer microorganisms for bioremediation ## When to use me - When developing recombinant protein production systems - When designing fermentation and bioprocessing - When applying CRISPR and genetic engineering - When creating diagnostic or therapeutic applications - When optimizing bioreactor conditions - When developing sustainable bioprocesses ## Key Concepts ### Genetic Engineering **Tools** - Restriction enzymes: DNA cleavage - Ligases: DNA joining - PCR: DNA amplification - CRISPR-Cas9: Genome editing - Transformation: Gene insertion ### Bioprocess Engineering ```python # Example: Simple batch fermentation model def batch_fermentation(X0, mu, t): """ Calculate cell concentration in batch culture. X0: Initial cell concentration mu: Specific growth rate (h^-1) t: Time (hours) """ return X0 * np.exp(mu * t) def yield_coefficient(S0, Xf, P): """Calculate biomass and product yields.""" Yx_s = (Xf - X0) / (S0 - Sf) # Biomass yield Yp_s = P / (S0 - Sf) # Product yield return Yx_s, Yp_s ``` ### Applications - Recombinant proteins: Insulin, antibodies, vaccines - Biopharmaceuticals: Therapeutic proteins, gene therapy - Industrial enzymes: Amylases, proteases, lipases - Biofuels: Ethanol, biodiesel, biogas - Bioremediation: Pollutant degradation - Agricultural biotechnology: GM crops, biofertilizers