| name | primer-design-check |
| description | Check primers for dimers, hairpins, and off-target amplification |
| version | 1.0.0 |
| category | Wet Lab |
| tags | [] |
| author | AIPOCH |
| license | MIT |
| status | Draft |
| risk_level | Medium |
| skill_type | Tool/Script |
| owner | AIPOCH |
| reviewer | |
| last_updated | 2026-02-06 |
Primer Design Check
In silico primer validation tool.
Use Cases
- qPCR primer design
- Sequencing primer check
- Mutagenesis primer validation
Parameters
forward_primer: F sequence
reverse_primer: R sequence
template: Target genome (optional)
Returns
- Dimer prediction
- Hairpin analysis
- Off-target BLAST results
- Tm and GC% calculations
Example
Flags: Self-dimer detected at 3' end → redesign recommended
Risk Assessment
| Risk Indicator | Assessment | Level |
|---|
| Code Execution | Python/R scripts executed locally | Medium |
| Network Access | No external API calls | Low |
| File System Access | Read input files, write output files | Medium |
| Instruction Tampering | Standard prompt guidelines | Low |
| Data Exposure | Output files saved to workspace | Low |
Security Checklist
Prerequisites
No additional Python packages required.
Evaluation Criteria
Success Metrics