| name | wb-troubleshooter |
| description | Western Blot troubleshooting guide with step-by-step solutions |
| version | 1.0.0 |
| category | Wet Lab |
| tags | [] |
| author | AIPOCH |
| license | MIT |
| status | Draft |
| risk_level | Medium |
| skill_type | Tool/Script |
| owner | AIPOCH |
| reviewer | |
| last_updated | 2026-02-06 |
WB Troubleshooter
Western Blot problem diagnosis and solutions.
Use Cases
- High background troubleshooting
- No signal diagnosis
- Multiple bands resolution
- Band smearing fixes
Parameters
symptom: Problem description
antibody_type: Primary/Secondary
Returns
- Systematic troubleshooting steps
- Likely causes ranked
- Solution recommendations
Example
Input: "High background"
Output: Check blocking buffer → antibody concentration → wash stringency
Risk Assessment
| Risk Indicator | Assessment | Level |
|---|
| Code Execution | Python/R scripts executed locally | Medium |
| Network Access | No external API calls | Low |
| File System Access | Read input files, write output files | Medium |
| Instruction Tampering | Standard prompt guidelines | Low |
| Data Exposure | Output files saved to workspace | Low |
Security Checklist
Prerequisites
No additional Python packages required.
Evaluation Criteria
Success Metrics