nta-particle-tracker

name	nta-particle-tracker
description	Nanoparticle Tracking Analysis skill for single-particle size and concentration measurements
allowed-tools	["Read","Write","Glob","Grep","Bash"]
metadata	{"specialization":"nanotechnology","domain":"science","category":"microscopy-characterization","priority":"medium","phase":6,"tools-libraries":["NanoSight NTA software","ZetaView software"]}
graph	{"domains":["domain:nanotechnology"],"skillAreas":["skill-area:mathematical-reasoning","skill-area:physics-simulation","skill-area:data-analysis"],"workflows":["workflow:experiment-design"],"roles":["role:research-engineer"]}

NTA Particle Tracker

Purpose

The NTA Particle Tracker skill provides single-particle tracking analysis for nanoparticle size and concentration measurements, offering particle-by-particle characterization through direct visualization.

Capabilities

• Single-particle tracking
• Size-concentration profiles
• Fluorescence NTA mode
• Real-time size distribution
• Particle count and concentration
• Sample heterogeneity assessment

Usage Guidelines

NTA Measurement

1. Sample Preparation

   • Dilute to 10^7-10^9 particles/mL
   • Avoid air bubbles
   • Use appropriate buffer

2. Acquisition

   • Optimize camera level
   • Set detection threshold
   • Record multiple videos

3. Analysis

   • Track minimum 1000 particles
   • Set minimum track length
   • Generate size-concentration plot

Process Integration

• Statistical Particle Size Distribution Analysis
• Nanoparticle Drug Delivery System Development

Input Schema

{
  "sample_id": "string",
  "mode": "scatter|fluorescence",
  "dilution_factor": "number",
  "capture_time": "number (seconds)",
  "number_of_captures": "number"
}

Output Schema

{
  "concentration": "number (particles/mL)",
  "mean_size": "number (nm)",
  "mode_size": "number (nm)",
  "d10": "number (nm)",
  "d50": "number (nm)",
  "d90": "number (nm)",
  "size_distribution": {"sizes": [], "counts": []},
  "particles_tracked": "number"
}