| name | geo-reanalysis |
| description | Guide reanalysis of GEO datasets with standard bioinformatics pipelines. |
GEO Reanalysis
Reanalyze public GEO datasets for validation or hypothesis testing.
Workflow
-
Identify Dataset
- Search GEO for relevant series (GSE)
- Check sample metadata and experimental design
- Verify platform and annotation
-
Download
- GEOquery (R) or GEOparse (Python)
- Raw data (CEL, FASTQ) or processed matrices
-
QC
- Sample clustering
- Batch effect assessment
- Outlier detection
-
Analysis
- Differential expression (limma, DESeq2, edgeR)
- Pathway enrichment
- Survival analysis (if clinical data available)
-
Validation
- Compare with original publication
- Cross-validate with independent cohort
Output
Save reanalysis log to reanalyses/geo-<slug>.md with:
- Dataset ID and description
- QC results
- Analysis parameters
- Key findings
- Comparison with original study