ihc-if-optimizer

name	ihc-if-optimizer
description	Optimize IHC/IF protocols for specific tissues and antigens
version	1.0.0
category	Wet Lab
tags	[]
author	AIPOCH
license	MIT
status	Draft
risk_level	Medium
skill_type	Tool/Script
owner	AIPOCH
reviewer
last_updated	2026-02-06

IHC/IF Optimizer

Immunostaining protocol optimization.

Use Cases

• Brain tissue staining
• Liver antigen retrieval
• Antibody dilution optimization
• Fluorescence panel design

Parameters

Parameter	Type	Default	Required	Description
--tissue-type	string	-	Yes	Tissue type (Brain, Liver, Kidney, etc.)
--antigen	string	-	Yes	Target protein/antigen name
--detection-method	string	IHC	No	Detection method (IHC or IF)
--output, -o	string	stdout	No	Output file path
--format	string	text	No	Output format (text, json, markdown)

Returns

• Recommended retrieval method
• Antibody dilutions
• Blocking conditions
• Counterstain suggestions

Example

Brain tissue + Phospho-protein → Citrate retrieval, 1:200 antibody

Risk Assessment

Risk Indicator	Assessment	Level
Code Execution	Python/R scripts executed locally	Medium
Network Access	No external API calls	Low
File System Access	Read input files, write output files	Medium
Instruction Tampering	Standard prompt guidelines	Low
Data Exposure	Output files saved to workspace	Low

Security Checklist

• No hardcoded credentials or API keys
• No unauthorized file system access (../)