primer-wetlab-qc

name	primer-wetlab-qc
description	Use when an agent must evaluate primer candidates for wet-lab constraints such as length, GC, Tm, degeneracy, self-complementarity, pair complementarity, and amplicon size.

Primer Wet-Lab QC

Use this skill after primer_site artifacts or external candidate generation. The bundled helper is dependency-free and conservative; final release should also run Primer3/ntthal or an equivalent thermodynamic tool when installed.

python agentskill/scripts/primer_panel_qc.py qc-primers \
  --primers primer_pairs.csv \
  --output primer_qc.json \
  --csv-output primer_qc.csv \
  --min-primer-len 18 \
  --max-primer-len 30 \
  --min-tm 50 \
  --max-tm 68 \
  --max-degeneracy 16

Review all pairs with:

• degeneracy_too_high;
• three_prime_*_complement_high;
• pair_complement_run_high;
• amplicon_length_out_of_range;
• large Tm differences between forward and reverse primers.

Treat primer_qc.json as a design gate before specificity screening and panel export.