| name | crispr-grna-designer |
| description | Design CRISPR gRNA sequences for specific gene exons with off-target prediction and efficiency scoring. Trigger when user needs gRNA design, CRISPR guide RNA selection, or genome editing target analysis. |
| version | 1.0.0 |
| category | Bioinfo |
| tags | ["crispr","grna","genome-editing","bioinformatics","off-target","cas9"] |
| author | AIPOCH |
| license | MIT |
| status | Draft |
| risk_level | High |
| skill_type | Hybrid (Tool/Script + Network/API) |
| owner | AIPOCH |
| reviewer | null |
| last_updated | 2026-02-06T00:00:00.000Z |
CRISPR gRNA Designer
Design optimal guide RNA (gRNA) sequences for CRISPR-Cas9 genome editing. Supports on-target efficiency scoring and off-target prediction.
Use Cases
- Design gRNAs for gene knockout (KO) experiments
- Select high-efficiency guides for specific exons
- Predict and minimize off-target effects
- Optimize for SpCas9, SpCas9-NG, xCas9 variants
Input Parameters
| Parameter | Type | Required | Description |
|---|
gene_symbol | string | Yes | HGNC gene symbol (e.g., TP53, BRCA1) |
target_exon | int | No | Specific exon number (default: all coding exons) |
genome_build | string | No | Reference genome: hg38 (default), hg19, mm10 |
pam_sequence | string | No | PAM motif: NGG (default), NAG, NGCG |
guide_length | int | No | gRNA length in bp (default: 20) |
gc_content_min | float | No | Minimum GC% (default: 30) |
gc_content_max | float | No | Maximum GC% (default: 70) |
poly_t_threshold | int | No | Max consecutive T's (default: 4) |
off_target_check | bool | No | Enable off-target prediction (default: true) |
max_mismatches | int | No | Max mismatches for off-target (default: 3) |
Output Format
{
"gene": "TP53",
"genome": "hg38",
...
