crispr-grna-designer

name	crispr-grna-designer
description	Design CRISPR gRNA sequences for specific gene exons with off-target prediction and efficiency scoring. Trigger when user needs gRNA design, CRISPR guide RNA selection, or genome editing target analysis.
version	1.0.0
category	Bioinfo
tags	["crispr","grna","genome-editing","bioinformatics","off-target","cas9"]
author	AIPOCH
license	MIT
status	Draft
risk_level	High
skill_type	Hybrid (Tool/Script + Network/API)
owner	AIPOCH
reviewer	null
last_updated	2026-02-06T00:00:00.000Z

CRISPR gRNA Designer

Design optimal guide RNA (gRNA) sequences for CRISPR-Cas9 genome editing. Supports on-target efficiency scoring and off-target prediction.

Use Cases

• Design gRNAs for gene knockout (KO) experiments
• Select high-efficiency guides for specific exons
• Predict and minimize off-target effects
• Optimize for SpCas9, SpCas9-NG, xCas9 variants

Input Parameters

Parameter	Type	Required	Description
gene_symbol	string	Yes	HGNC gene symbol (e.g., TP53, BRCA1)
target_exon	int	No	Specific exon number (default: all coding exons)
genome_build	string	No	Reference genome: hg38 (default), hg19, mm10
pam_sequence	string	No	PAM motif: NGG (default), NAG, NGCG
guide_length	int	No	gRNA length in bp (default: 20)
gc_content_min	float	No	Minimum GC% (default: 30)
gc_content_max	float	No	Maximum GC% (default: 70)
poly_t_threshold	int	No	Max consecutive T's (default: 4)
off_target_check	bool	No	Enable off-target prediction (default: true)
max_mismatches	int	No	Max mismatches for off-target (default: 3)

Output Format

{
  "gene": "TP53",
  "genome": "hg38",
   
    
       
       
       
       
       
       
       
       
       
       ...