| name | serial-dilution-calculator |
| description | Generate qPCR/ELISA dilution protocols with precise pipetting steps |
| version | 1.0.0 |
| category | Wet Lab |
| tags | [] |
| author | AIPOCH |
| license | MIT |
| status | Draft |
| risk_level | Medium |
| skill_type | Tool/Script |
| owner | AIPOCH |
| reviewer | |
| last_updated | 2026-02-06 |
Serial Dilution Calculator
Step-by-step dilution protocol generator.
Use Cases
- qPCR standard curves
- ELISA plate setup
- Drug dose responses
- MIC determinations
Parameters
starting_conc: Stock concentration
final_conc: Target concentration
dilution_factor: Step dilution
total_volume: Per well volume
Returns
- Pipetting scheme table
- Required volumes
- Plate layout suggestion
- Common pitfall warnings
Example
"Take 10uL stock + 90uL diluent for 1:10..."
Risk Assessment
| Risk Indicator | Assessment | Level |
|---|
| Code Execution | Python/R scripts executed locally | Medium |
| Network Access | No external API calls | Low |
| File System Access | Read input files, write output files | Medium |
| Instruction Tampering | Standard prompt guidelines | Low |
| Data Exposure | Output files saved to workspace | Low |
Security Checklist
Prerequisites
No additional Python packages required.