serial-dilution-calculator

name	serial-dilution-calculator
description	Generate qPCR/ELISA dilution protocols with precise pipetting steps
version	1.0.0
category	Wet Lab
tags	[]
author	AIPOCH
license	MIT
status	Draft
risk_level	Medium
skill_type	Tool/Script
owner	AIPOCH
reviewer
last_updated	2026-02-06

Serial Dilution Calculator

Step-by-step dilution protocol generator.

Use Cases

• qPCR standard curves
• ELISA plate setup
• Drug dose responses
• MIC determinations

Parameters

• starting_conc: Stock concentration
• final_conc: Target concentration
• dilution_factor: Step dilution
• total_volume: Per well volume

Returns

• Pipetting scheme table
• Required volumes
• Plate layout suggestion
• Common pitfall warnings

Example

"Take 10uL stock + 90uL diluent for 1:10..."

Risk Assessment

Risk Indicator	Assessment	Level
Code Execution	Python/R scripts executed locally	Medium
Network Access	No external API calls	Low
File System Access	Read input files, write output files	Medium
Instruction Tampering	Standard prompt guidelines	Low
Data Exposure	Output files saved to workspace	Low

Security Checklist

• No hardcoded credentials or API keys
• No unauthorized file system access (../)
• Output does not expose sensitive information
• Prompt injection protections in place
• Input file paths validated (no ../ traversal)
• Output directory restricted to workspace
• Script execution in sandboxed environment
• Error messages sanitized (no stack traces exposed)
• Dependencies audited

Prerequisites

No additional Python packages required.