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SkillsMP hat 604 Skills aus PKU-YuanGroup/OpenAI4S gesammelt. Öffne einen Skill, um Quelle und Details zu prüfen.
PKU-YuanGroup/OpenAI4SEs werden 40 von 604 gesammelten Skills angezeigt.
Transcribe DNA to RNA and translate to protein using Biopython, with NCBI codon-table selection, CDS validation, and six-frame ORF finding. Use when converting a CDS or ORF to its amino-acid sequence, selecting a non-standard (mitochondrial, bacterial,…
Quellsprache: Englisch
Integrate multiple scRNA-seq samples or batches with Harmony, scVI/scANVI, Seurat (CCA/RPCA), fastMNN, Scanorama, or BBKNN. Resolves which method to use for the dataset size and design, how strongly to correct, when integration is the wrong move (confounded…
Quellsprache: Englisch
Automated reference-based cell type annotation for single-cell RNA-seq using CellTypist, SingleR, Azimuth, scANVI, and scmap to transfer labels from a reference. Use when annotating cell types from a reference atlas or pretrained model, transferring labels…
Quellsprache: Englisch
Infers ligand-receptor cell-cell communication from scRNA-seq with a consensus-first workflow (LIANA), plus CellPhoneDB specificity tests, CellChat pathway probabilities, and NicheNet downstream ligand-activity. Use when ranking ligand-receptor interactions…
Quellsprache: Englisch
Dimensionality reduction and graph-based clustering for single-cell RNA-seq with Scanpy (Python) and Seurat (R). Resolves which algorithm to use (Leiden vs Louvain), how many PCs and neighbors to set, how to sweep and validate resolution, when a split is…
Quellsprache: Englisch
Infer large-scale copy-number alterations from tumor single-cell or single-nucleus RNA-seq to separate malignant from normal cells and call subclones, using inferCNV, copyKAT, Numbat, and SCEVAN. Use when separating malignant from normal cells in a tumor…
Quellsprache: Englisch
Read, write, create, and convert single-cell objects across AnnData (Python), Seurat (R), and SingleCellExperiment (R). Use when loading 10X Cell Ranger output (raw vs filtered), importing or exporting h5ad/RDS/h5mu/zarr, building AnnData or Seurat objects…
Quellsprache: Englisch
Test whether cell-type proportions or composition changed between conditions in single-cell data using Milo (miloR), scCODA, sccomp, and propeller. Use when comparing cell-type proportions / composition between conditions, asking which populations expanded or…
Quellsprache: Englisch
Detect and remove doublets (two or more cells in one droplet) from single-cell RNA-seq using scDblFinder (R), Scrublet (Python), and DoubletFinder (R). Use when flagging artificial intermediate populations before clustering, setting the expected doublet rate…
Quellsprache: Englisch
Assign cells to their sample of origin from cell or nucleus hashing (CITE-seq HTOs, MULTI-seq lipid/cholesterol tags, CellPlex CMOs) and call cross-sample doublets using Seurat HTODemux/MULTIseqDemux, hashsolo, demuxEM, GMM-Demux, and demuxmix. Use when…
Quellsprache: Englisch
Reconstructs single-cell lineage trees and clonal relationships from CRISPR/Cas9 scars, static expressed barcodes (LARRY/CellTag), or somatic mtDNA mutations using Cassiopeia, Startle, and CoSpar. Use when building a phylogeny from barcode scars, choosing a…
Quellsprache: Englisch
Detect cluster marker genes and assign manual cell type labels in single-cell RNA-seq using Scanpy (Python) and Seurat (R). Use when finding genes that distinguish clusters, ranking markers for annotation, scoring gene signatures, hand-labeling clusters, or…
Quellsprache: Englisch
Infers metabolite-mediated cell-cell communication from scRNA-seq by scoring enzyme-to-sensor pairs (MEBOCOST), with metabolic flux (scFEA), FBA state (Compass), and neurotransmitter (NeuronChat) alternatives. Use when studying metabolic crosstalk between…
Quellsprache: Englisch
Integrate multimodal single-cell data (CITE-seq RNA+protein, 10x Multiome RNA+ATAC, unpaired/diagonal RNA+ATAC) and choose the right joint method. Use when classifying an integration task by anchor structure (paired vs unpaired), denoising CITE-seq ADT…
Quellsprache: Englisch
Analyze Perturb-seq / CROP-seq single-cell CRISPR screens. Use when assigning guides as a mixture problem, removing non-perturbed escaper cells with Mixscape, choosing a calibrated test (SCEPTRE conditional resampling) over naive DE, quantifying effect size…
Quellsprache: Englisch
Quality control, ambient-RNA handling, normalization, and feature selection for single-cell RNA-seq using Scanpy (Python) and Seurat (R). Use when filtering low-quality cells with MAD-adaptive thresholds, setting tissue-aware mito cutoffs, removing ambient…
Quellsprache: Englisch
Analyze single-cell ATAC-seq with Signac/ArchR (R) and SnapATAC2 (Python alternative). Use when processing scATAC fragments, choosing a framework, calling consensus peaks, running TF-IDF/LSI while diagnosing the depth component, scoring chromVAR motif…
Quellsprache: Englisch
Infers developmental trajectories, pseudotime, RNA velocity, and directed fate probabilities from single-cell data using PAGA, Slingshot, Monocle3, DPT, Palantir, scVelo, and CellRank 2. Use when ordering cells along a differentiation continuum, choosing a…
Quellsprache: Englisch
Tests miRNAs for differential expression with DESeq2 or edgeR using small-RNA-aware normalization and filtering. Use when deciding which normalization survives a library dominated by a few hyper-abundant miRNAs (compositional fragility); choosing DESeq2 vs…
Quellsprache: Englisch
Discovers novel miRNAs and quantifies known miRNAs with miRDeep2 by scoring genome-mapped read stacks against the Dicer/Drosha biogenesis signature. Use when deciding whether a study needs de novo discovery at all versus known-miRNA quantification; choosing…
Quellsprache: Englisch
Quantifies known miRNAs, isomiRs, tRFs, and A-to-I editing fast with miRge3.0 by aligning collapsed reads to curated miRBase or MirGeneDB libraries. Use when choosing miRBase versus MirGeneDB as the reference; deciding whether to collapse isomiRs to the…
Quellsprache: Englisch
Trims kit-specific 3' adapters, strips UMIs or 4N degenerate ends, size-selects, and collapses small RNA-seq reads (miRNA, piRNA, tRF) with cutadapt or fastp. Use when choosing the kit's 3' adapter; setting the size window (18-26 nt miRNA vs 24-32 nt piRNA);…
Quellsprache: Englisch
Predicts and prioritizes miRNA target genes with seed-based tools (miRanda, TargetScan, miRDB) and experimentally validated databases (miRTarBase, multiMiR). Use when deciding that a predicted target is a hypothesis not a finding; ranking by the right score…
Quellsprache: Englisch
Profiles non-miRNA small RNAs - tRNA-derived fragments (tRFs/tsRNAs), piRNAs, and rRNA/snoRNA-derived species - with MINTmap, unitas, SPORTS, and proTRAC. Use when annotating all small-RNA classes in a library; quantifying tRFs at locus resolution where tRNA…
Quellsprache: Englisch
Reconstructs single cells from sub-cellular spatial capture units (Visium HD 2um bins, Stereo-seq DNB spots, Slide-seqV2 beads) by aggregating bins UP into cells rather than deconvolving a mixture DOWN. Use when choosing a bin size and recognizing the…
Quellsprache: Englisch
Segments cells/nuclei and extracts image features from imaging spatial transcriptomics (Xenium, MERFISH/MERSCOPE, CosMx) and H&E/IF tissue images using Cellpose, StarDist, Baysor, and Squidpy. Use when choosing a segmentation strategy (DAPI nucleus +…
Quellsprache: Englisch
Maps cell-cell communication and ligand-receptor co-expression in spatial transcriptomics (Visium, Xenium, MERFISH, CosMx, Slide-seq) with Squidpy ligrec, COMMOT, stLearn, CellChat-spatial, and NicheNet. Use when choosing a method by whether spatial distance…
Quellsprache: Englisch
Loads spatial transcriptomics data from Visium, Visium HD, Xenium, MERFISH/MERSCOPE, CosMx, Slide-seq/Curio, and Stereo-seq into AnnData or SpatialData using spatialdata-io and Squidpy. Use when deciding which platform class is in hand (imaging/in-situ vs…
Quellsprache: Englisch
Estimates per-spot cell type composition of spatial transcriptomics mixtures (Visium, Slide-seq, Stereo-seq) from an scRNA-seq reference with cell2location, RCTD, SPOTlight, stereoscope, SpatialDWLS, or reference-free STdeconvolve. Use when deciding whether a…
Quellsprache: Englisch
Identify spatially coherent tissue domains (regions like cortical layers, tumor vs stroma) in Visium, Visium HD, Xenium, MERFISH, Slide-seq, and Stereo-seq data with Squidpy, BANKSY, BayesSpace, STAGATE, and GraphST. Use when distinguishing a domain (a region…
Quellsprache: Englisch
Integrates spatial RNA with a second modality (protein, ATAC, or histone marks) on spatial CITE-seq, DBiT-seq, spatial-ATAC, or Visium CytAssist data. Use when deciding vertical (same-pixel co-profiling -> WNN/MOFA joint factors) versus diagonal (serial…
Quellsprache: Englisch
Build the spatial neighbor graph that every downstream spatial statistic (Moran's I, neighborhood enrichment, co-occurrence, spatial domains) inherits, using Squidpy. Use when choosing the graph type (kNN vs Delaunay vs fixed-radius vs Visium hex grid) and…
Quellsprache: Englisch
Quality control, filtering, and normalization for spatial transcriptomics (Visium, Visium HD, Xenium, MERFISH/MERSCOPE, CosMx, Slide-seq) with Squidpy and Scanpy. Use when setting QC floors that do NOT delete real low-count imaging cells (an scRNA…
Quellsprache: Englisch
Analyzes multiplexed antibody-imaging data (CODEX/PhenoCycler, MIBI-TOF, IMC, CyCIF, Opal/Vectra mIF) as continuous protein intensity rather than transcript counts, using scimap and squidpy. Use when choosing an intensity transform/normalization (arcsinh…
Quellsprache: Englisch
Detects spatially variable genes, spatial autocorrelation, and cell-type colocalization for spatial transcriptomics using Squidpy with PySAL/esda for local statistics. Use when choosing an SVG method by its null and scaling (SpatialDE/SPARK GP…
Quellsprache: Englisch
Plots spatial transcriptomics expression, clusters, and annotations on tissue using Squidpy and Scanpy. Use when choosing the plotter and spot size by platform fork (sc.pl.spatial / sq.pl.spatial_scatter with real scalefactors and capture diameter for…
Quellsprache: Englisch
Retrieves and interprets AlphaFold Protein Structure Database (AFDB) models by UniProt accession, reading pLDDT and PAE confidence correctly. Use when treating pLDDT as PER-RESIDUE confidence (not global accuracy) and recognizing a long low-pLDDT stretch as…
Quellsprache: Englisch
Detects putative ligand-binding pockets and druggable cavities de novo on an apo protein structure with fpocket, P2Rank, CASTp, and DoGSiteScorer, ranking them by druggability/ligandability score. Use when detecting cavities on an apo structure with no bound…
Quellsprache: Englisch
Measures geometric properties of protein structures with Biopython Bio.PDB - interatomic distances, distance matrices, bond and dihedral angles (phi/psi/chi, Ramachandran), superposition and RMSD, center of mass, radius of gyration, and solvent accessible…
Quellsprache: Englisch
Maps protein-protein and protein-ligand interfaces with Bio.PDB, computing contact residues and buried surface area (BSA). Use when choosing a contact cutoff and stating its rationale (heavy-atom 4-5A vs CA-CA 8A vs a SASA-based definition); deciding a…
Quellsprache: Englisch