| name | barrnap |
| description | Basic rapid ribosomal RNA predictor for genomes. Use this skill when working with barrnap: installing, choosing parameters, running commands, interpreting outputs, or troubleshooting. Upstream: https://github.com/tseemann/barrnap. For stage routing use tool-selection / metagenomics-workflow.
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| license | MIT |
| category | analysis-tools |
| tags | ["rrna","annotation"] |
| upstream | https://github.com/tseemann/barrnap |
| stage | gene |
Barrnap
Upstream: Barrnap
Citation
Seemann, T. Barrnap: BAsic Rapid Ribosomal RNA Predictor. https://github.com/tseemann/barrnap
See also docs/references.md.
Analytical thinking
Quick rRNA loci on MAGs/isolates for QC (16S presence) and annotation. Pair with GTDB-Tk / Prokka workflows.
How to run
conda install -c bioconda barrnap
barrnap bins/bin.fa > temp/barrnap/${id}.gff
Key parameters
| kingdom | bac/arc/euk | |
Decision notes
- Stage: function (rRNA prediction)
- Prefer as a quick rRNA check on genomes/MAGs; not a substitute for full annotation (
bakta/prokka)
Related skills
prokka · gtdbtk · checkm2 · tool-selection · metagenomics-workflow