barrnap

name	barrnap
description	Basic rapid ribosomal RNA predictor for genomes. Use this skill when working with barrnap: installing, choosing parameters, running commands, interpreting outputs, or troubleshooting. Upstream: https://github.com/tseemann/barrnap. For stage routing use tool-selection / metagenomics-workflow.
license	MIT
category	analysis-tools
tags	["rrna","annotation"]
upstream	https://github.com/tseemann/barrnap
stage	gene

Barrnap

Upstream: Barrnap

Citation

Seemann, T. Barrnap: BAsic Rapid Ribosomal RNA Predictor. https://github.com/tseemann/barrnap

See also docs/references.md.

Analytical thinking

Quick rRNA loci on MAGs/isolates for QC (16S presence) and annotation. Pair with GTDB-Tk / Prokka workflows.

How to run

conda install -c bioconda barrnap  # if packaged; else follow upstream

barrnap bins/bin.fa > temp/barrnap/${id}.gff

Key parameters

| kingdom | bac/arc/euk | |

Decision notes

• Stage: function (rRNA prediction)
• Prefer as a quick rRNA check on genomes/MAGs; not a substitute for full annotation (bakta/prokka)

Related skills

prokka · gtdbtk · checkm2 · tool-selection · metagenomics-workflow